Human CCL24/Eotaxin-2/MPIF-2 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0283

Human CCL24/Eotaxin-2/MPIF-2 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

31.25~2000 pg/ml

Overview

Product Name

Human CCL24/Eotaxin-2/MPIF-2 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.75 pg/ml (50 μl)；9.71 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0422	0.0372	0.0397
31.25	0.0914	0.0879	0.0897	0.0500
62.50	0.1376	0.1466	0.1421	0.1024
125.00	0.2545	0.2710	0.2628	0.2231
250.00	0.5244	0.5137	0.5191	0.4794
500.00	1.0090	1.0250	1.0170	0.9773
1000.00	2.0390	2.0250	2.0320	1.9923
2000.00	3.3060	3.3210	3.3135	3.2738

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	341.7	1933.2	5656.0	396.7	1924.8	5724.1
Standard Deviation	25.4	50.6	153.1	30.8	36.8	93.0
Coefficient of Variation (%)	7.4	2.6	2.7	7.8	1.9	1.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CCL24/Eotaxin-2/MPIF-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 105% to 126% with an overall mean recovery of 108%.

Sample Value

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	137.98-767.80	100.0	377.41

Serum/Plasma– Thirty samples from apparently healthy volunteers were evaluated for the presence of human CCL24/Eotaxin-2/MPIF-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: O00175 Human

Function

Eotaxin, also named MPIF-2 and Ck beta 6, is a novel CC chemokine identified in the Human Genome Sciences, Inc. database based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin-2 cDNA encodes a 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. Although one recombinant preparation of MPIF‑2/Eotaxin‑2 from insect cells was reported to be glycosylated and of the predicted size (10.5 kDa), a second preparation of recombinant Eotaxin-2 prepared by a different group, also from insect cells, was shown to contain a 78 amino acid residue carboxy-terminally truncated variant of Eotaxin-2. Additional minor carboxy‑terminally truncated variants with 73, 75 and 76 residues were also isolated. Compared to other CC chemokines, Eotaxin-2 exhibits 40%, 42% and 39% amino acid identity to MCP-3, MIP-1 alpha, and Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 has been shown to induce chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin-2 has also been shown to suppress the colony formation by the high proliferative potential colony-forming cells which represent multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2 was shown to be inhibited by an anti-CCR-3 antibody and to be cross-desensitized by Eotaxin or MCP-4, suggesting that all three CC chemokines act through CCR-3, at least on eosinophils.

Human CCL24/Eotaxin-2/MPIF-2 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Function

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