Human CD127/IL-7 R alpha enzyme-linked immunoassay kit
Catalog# EH0259
Human CD127/IL-7 R alpha enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
Overview
Product Name
Human CD127/IL-7 R alpha enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
10.55 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0640 | 0.0630 | 0.0635 | |
| 156.25 | 0.1851 | 0.1668 | 0.1760 | 0.1125 |
| 312.50 | 0.4100 | 0.3861 | 0.3981 | 0.3346 |
| 625.00 | 0.7055 | 0.6823 | 0.6939 | 0.6304 |
| 1250.00 | 1.2610 | 1.2630 | 1.2620 | 1.1985 |
| 2500.00 | 2.0830 | 2.0410 | 2.0620 | 1.9985 |
| 5000.00 | 3.1730 | 3.1000 | 3.1365 | 3.0730 |
| 10000.00 | 4.1100 | 4.0396 | 4.0748 | 4.0113 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 196.2 | 1212.5 | 3366.8 | 200.9 | 1152.1 | 3404.0 |
| Standard Deviation | 6.7 | 40.4 | 193.6 | 6.5 | 57.4 | 147.8 |
| Coefficient of Variation (%) | 3.4 | 3.3 | 5.8 | 3.2 | 5.0 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Human CD127/IL-7 R alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 74% to 107% with an overall mean recovery of 93%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4551.82-11441.82 | 100 | 7455.88 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Human CD127/IL-7 R alpha in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃