Human BCMA/TNFRSF17 enzyme-linked immunoassay kit
Catalog# EH0143
Human BCMA/TNFRSF17 enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
Overview
Product Name
Human BCMA/TNFRSF17 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.45 pg/ml (50 μl)；1.52 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0088 | 0.0086 | 0.0087 | |
| 6.86 | 0.0244 | 0.0247 | 0.0246 | 0.0158 |
| 20.58 | 0.0611 | 0.0643 | 0.0627 | 0.0540 |
| 61.73 | 0.2083 | 0.2159 | 0.2121 | 0.2034 |
| 185.19 | 0.6262 | 0.6201 | 0.6232 | 0.6144 |
| 555.56 | 1.8500 | 1.9220 | 1.8860 | 1.8773 |
| 1666.67 | 3.2090 | 3.2870 | 3.2480 | 3.2393 |
| 5000.00 | 3.6870 | 3.6950 | 3.6910 | 3.6823 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 97.3 | 531.3 | 963.5 | 102.3 | 532.2 | 1496.3 |
| Standard Deviation | 3.7 | 26.4 | 17.4 | 1.9 | 16.1 | 28.5 |
| Coefficient of Variation (%) | 3.8 | 5.0 | 1.8 | 1.9 | 3.0 | 1.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human BCMA/TNFRSF17 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 106% with an overall mean recovery of 93%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1715.39-3905.61 | 100 | 2432.79 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of BCMA/TNFRSF17 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
B cell maturation antigen
B cell maturation factor
B cell maturation protein
B-cell maturation protein
BCM
BCMA
CD269
CD269 antigen
TNFRSF17
TNR17_HUMAN
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