Human GM-CSF enzyme-linked immunoassay kit
Catalog# EH0051
Human GM-CSF enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
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unconjugated
Overview
Product Name
Human GM-CSF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.57 pg/ml (50 μl)；5.45 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0082 | 0.0082 | 0.0082 | |
| 13.72 | 0.0260 | 0.0266 | 0.0263 | 0.0181 |
| 41.15 | 0.0637 | 0.0699 | 0.0668 | 0.0586 |
| 123.46 | 0.1806 | 0.1768 | 0.1787 | 0.1705 |
| 370.37 | 0.4666 | 0.4923 | 0.4795 | 0.4713 |
| 1111.11 | 1.2090 | 1.2760 | 1.2425 | 1.2343 |
| 3333.33 | 2.3060 | 2.5770 | 2.4415 | 2.4333 |
| 10000.00 | 3.3860 | 3.5690 | 3.4775 | 3.4693 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 195 | 895.9 | 2513.3 | 183.5 | 915.4 | 2844.3 |
| Standard Deviation | 11.9 | 43.4 | 183.3 | 10.9 | 45.6 | 159.5 |
| Coefficient of Variation (%) | 6.1 | 4.8 | 7.3 | 5.9 | 5.0 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human GM-CSF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 75% to 100% with an overall mean recovery of 84%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-3.96 | 21 | 3.02 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-8 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CSF
CSF2
GMCSF
GM-CSF
colony stimulating factor 2 (granulocyte-macrophage)
Colony-stimulating factor
GMCSFgranulocyte-macrophage colony-stimulating factor
granulocyte-macrophage colony stimulating factor
MGC131935
MGC138897
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