Human IL-3 enzyme-linked immunoassay kit
Catalog# EH0050
Human IL-3 enzyme-linked immunoassay kit
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ELISA
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Human
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40.96~10000 pg/ml
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unconjugated
Overview
Product Name
Human IL-3 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.74 pg/ml (50 μl)；20.64 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0157 | 0.0159 | 0.0158 | |
| 40.96 | 0.0347 | 0.0360 | 0.0354 | 0.0240 |
| 102.40 | 0.0625 | 0.0650 | 0.0638 | 0.0488 |
| 256.00 | 0.1395 | 0.1391 | 0.1393 | 0.1243 |
| 640.00 | 0.3246 | 0.3103 | 0.3175 | 0.3025 |
| 1600.00 | 0.8032 | 0.8016 | 0.8024 | 0.7874 |
| 4000.00 | 1.9100 | 1.9030 | 1.9065 | 1.8915 |
| 10000.00 | 3.2770 | 3.1920 | 3.2345 | 3.2195 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 244.6 | 1239.7 | 3546.7 | 191.8 | 849.4 | 2573.0 |
| Standard Deviation | 21.5 | 110.5 | 309.9 | 7.3 | 31.2 | 141.2 |
| Coefficient of Variation (%) | 8.8 | 8.9 | 8.7 | 3.8 | 3.7 | 5.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-3，未加人 IL-3 的血清作为本底，计算回收率。回收率的范围从 165 % 至 197 %，平均回收率为 184 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-40.96 | 37 | 20.6 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Colony stimulating factor multiple
Hematopoietic growth factor
IL 3
IL-3
IL3
IL3_HUMAN
Interleukin 3 (colony stimulating factor, multiple)
Interleukin 3
Interleukin-3
Mast cell growth factor
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