Mouse CD80/B7-1 enzyme-linked immunoassay kit
Catalog# EM0086
Mouse CD80/B7-1 enzyme-linked immunoassay kit
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ELISA
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Human
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78.13 ~5000 pg/ml
Overview
Product Name
Mouse CD80/B7-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.09pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0267 | 0.0271 | 0.0269 | |
| 78.13 | 0.1504 | 0.1522 | 0.1513 | 0.1244 |
| 156.25 | 0.2469 | 0.2433 | 0.2451 | 0.2182 |
| 312.50 | 0.4348 | 0.4403 | 0.4376 | 0.4107 |
| 625.00 | 0.8797 | 0.7761 | 0.8279 | 0.8010 |
| 1250.00 | 1.2430 | 1.3550 | 1.2990 | 1.2721 |
| 2500.00 | 2.0600 | 2.0030 | 2.0315 | 2.0046 |
| 5000.00 | 2.6530 | 2.6630 | 2.6580 | 2.6311 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 51.6 | 404.7 | 1626.5 | 86.6 | 518.6 | 1395.4 |
| Standard Deviation | 3.5 | 30.3 | 124.5 | 6.3 | 14.0 | 33.0 |
| Coefficient of Variation (%) | 6.8 | 7.5 | 7.7 | 7.3 | 2.7 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CD80/B7-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 120% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 602.90-820.21 | 100 | 680.63 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of mouse CD80/B7-1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃