Mouse CD163 enzyme-linked immunoassay kit（one step）
Catalog# EMY0087
Mouse CD163 enzyme-linked immunoassay kit（one step）
-
sELISA
-
Mouse
-
15.63~1000 pg/ml
Overview
Product Name
Mouse CD163 enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.02ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0058 | 0.0062 | 0.0060 | |
| 15.63 | 0.0399 | 0.0316 | 0.0358 | 0.0298 |
| 31.25 | 0.0761 | 0.0849 | 0.0805 | 0.0745 |
| 62.50 | 0.1626 | 0.1407 | 0.1517 | 0.1457 |
| 125.00 | 0.3227 | 0.3244 | 0.3236 | 0.3176 |
| 250.00 | 0.6385 | 0.6747 | 0.6566 | 0.6506 |
| 500.00 | 1.1360 | 1.2650 | 1.2005 | 1.1945 |
| 1000.00 | 2.6280 | 2.6700 | 2.6490 | 2.6430 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 28.3 | 157.5 | 346.9 | 34.0 | 149.5 | 345.8 |
| Standard Deviation | 2.2 | 11.2 | 15.6 | 1.7 | 11.1 | 18.9 |
| Coefficient of Variation (%) | 7.7 | 7.1 | 4.5 | 5.0 | 7.4 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CD163 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 58.63-152.00 | 100 | 80.70 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of mouse CD163 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD 163
CD163
CD163 antigen
CD163 molecule
Hemoglobin scavenger receptor
M130
M130 antigen precursor
Macrophage associated antigen
MM130
OTTHUMP00000238617
Expand