Mouse IL-17F enzyme-linked immunoassay kit
Catalog# EM0061
Mouse IL-17F enzyme-linked immunoassay kit
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ELISA
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Mouse
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4.10~1000 pg/ml
Overview
Product Name
Mouse IL-17F enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.26 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0253 | 0.0255 | 0.0254 | |
| 4.10 | 0.0690 | 0.0640 | 0.0665 | 0.0411 |
| 10.24 | 0.1212 | 0.1213 | 0.1213 | 0.0959 |
| 25.60 | 0.2649 | 0.2702 | 0.2676 | 0.2422 |
| 64.00 | 0.6330 | 0.6125 | 0.6228 | 0.5974 |
| 160.00 | 1.4450 | 1.3770 | 1.4110 | 1.3856 |
| 400.00 | 2.8630 | 2.8770 | 2.8700 | 2.8446 |
| 1000.00 | 4.3400 | 4.3574 | 4.3487 | 4.3233 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 19.3 | 89.6 | 301.5 | 19.5 | 93.9 | 315.2 |
| Standard Deviation | 0.6 | 2.8 | 12.7 | 0.7 | 2.2 | 3.8 |
| Coefficient of Variation (%) | 3.1 | 3.1 | 4.2 | 3.7 | 2.3 | 1.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-17F into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 115% with an overall mean recovery of 88%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.75-41.06 | 100 | 20.73 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-17F in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃