Mouse IL-7 enzyme-linked immunoassay kit
Catalog# EM0050
Mouse IL-7 enzyme-linked immunoassay kit
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ELISA
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Mouse
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2.74~2000 pg/ml
Overview
Product Name
Mouse IL-7 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.22 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0106 | 0.0105 | 0.0106 | |
| 20.83 | 0.0248 | 0.0245 | 0.0247 | 0.0141 |
| 41.67 | 0.0517 | 0.0515 | 0.0516 | 0.0411 |
| 83.33 | 0.1378 | 0.1363 | 0.1371 | 0.1265 |
| 166.67 | 0.3766 | 0.3879 | 0.3823 | 0.3717 |
| 333.33 | 1.0650 | 1.0660 | 1.0655 | 1.0550 |
| 666.67 | 2.5270 | 2.5730 | 2.5500 | 2.5395 |
| 2000.00 | 4.2655 | 4.2883 | 4.2770 | 4.2664 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 52.14 | 239.25 | 707.67 | 50.43 | 233.47 | 711.65 |
| Standard Deviation | 2.96 | 9.18 | 26.13 | 1.98 | 11.40 | 36.49 |
| Coefficient of Variation (%) | 5.7 | 3.8 | 3.7 | 3.9 | 4.9 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-7 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 110% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.6-6.24 | 100 | 2.98 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-7 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
IL 7
IL-7
Il7
IL7_HUMAN
Interleukin 7
Interleukin-7
LP-1
Lymphopoietin 1