Mouse TNF-α enzyme-linked immunoassay kit
Catalog# EM0010
Mouse TNF-α enzyme-linked immunoassay kit
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ELISA
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Mouse
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1.37~1000 pg/ml
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unconjugated
Overview
Product Name
Mouse TNF-α enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.097 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0121 | 0.0111 | 0.0116 | |
| 1.37 | 0.0242 | 0.0223 | 0.0233 | 0.0117 |
| 4.12 | 0.0482 | 0.0444 | 0.0463 | 0.0347 |
| 12.35 | 0.1134 | 0.1092 | 0.1113 | 0.0997 |
| 37.04 | 0.3165 | 0.3032 | 0.3099 | 0.2983 |
| 111.11 | 0.8042 | 0.7996 | 0.8019 | 0.7903 |
| 333.33 | 1.9672 | 1.9564 | 1.9618 | 1.9502 |
| 1000 | 4.1683 | 4.2715 | 4.2199 | 4.2083 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 16.1 | 80.4 | 271.7 | 14 | 75.2 | 236.7 |
| Standard Deviation | 1.1 | 4.8 | 16 | 0.3 | 4 | 10.8 |
| Coefficient of Variation (%) | 6.5 | 5.9 | 5.9 | 2 | 5.3 | 4.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠TNF-α，未加小鼠 TNF-α 的血清作为本底，计算回收率。
回收率的范围从 107 % 至 132 %，平均回收率为 116 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.28 | 33 | 0.16 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
Application in Publications
Target
UNIPROT
Alias
APC1
APC1 protein
Cachectin
DIF
Differentiation inducing factor
Macrophage cytotoxic factor
Tnf
TNF superfamily, member 2
TNF, macrophage derived
TNF, monocyte derived
ExpandFunction
Citation
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Colonic Engyodontium fungus triggers neutrophil antimicrobial activity to suppress Lactobacillus johnsonii-derived glutamic acid-maintained Tregs
Journal: Journal of Clinical Investigation
DOI: 10.1172/JCI196788
IF: 13.6
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Mar
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Mechanistic study of swertiamarin in treating ulcerative colitis by regulating YAP-mediated Wnt and Notch pathways for intestinal stem cell repair
Journal: Molecular Immunology
DOI: 10.1016/j.molimm.2026.02.012
IF: 3
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Mar
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Engineered M2 macrophage-derived vesicles deliver DNase I for cfDNA clearance and multi-organ protection in sepsis
Journal: International Journal of Pharmaceutics: X
DOI: 10.1016/j.ijpx.2026.100528
IF: 6.4
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Mar
-
Abolishing PorB-induced mitophagy enhances gonococcal outer membrane vesicle vaccine efficacy
Journal: Emerging Microbes & Infections
DOI: 10.1080/22221751.2026.2651468
IF: 7.5
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Mar
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TRIM21 Exacerbates Ischemic Brain Injury by Promoting Astrocyte-Mediated Neuroinflammation via K63-Linked Ubiquitination of MDA5
Journal: Research
DOI: 10.34133/research.1200
IF: 10.7
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Mar
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METTL3-mediated m6A on nascent RNA coordinates translational and transcriptional programs to activate the NLRP3 inflammasome in macrophages
Journal: Cell Reports
DOI: 10.1016/j.celrep.2025.116808
IF: 6.9
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Jan
-
Investigating the role of SMOX in rheumatoid arthritis
Journal: Molecular Immunology
DOI: 10.1016/j.molimm.2025.11.018
IF: 3
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Jan
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Functional Restoration of Uterine Architecture and Fertility via a 3D-Printed Biomimetic Scaffold: The Role of Macrophage-Driven Immunomodulation
Journal: Materials Today Bio
DOI: 10.1016/j.mtbio.2026.102935
IF: 10.2
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Feb
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A novel DYRK1A inhibitor alleviates lipopolysaccharide-induced acute lung injury via suppressing inflammatory pathway
Journal: International Immunopharmacology
DOI: 10.1016/j.intimp.2026.116300
IF: 4.7
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Feb
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Harmine derivative ZLWH-38 ameliorates the therapeutic efficacy of colistin against XDR-Ab pneumonia by suppressing inflammatory responses
Journal: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.115438
IF: 4.7
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Sept
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Phytochemicals From Hansenia weberbaueriana for Psoriasis and Inflammatory Bowel Disease Comorbidity: Based on Network Pharmacology and In Vitro Analysis
Journal: Chemistry & Biodiversity
DOI: 10.1002/cbdv.202502476
IF: 2.5
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Oct
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Lipocalin 2 deficiency attenuates NLRP3 inflammasome activation through glycolysis impairment and MGST1-mediated mitochondrial ROS reduction
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.149021
IF: 8.5
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Nov
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Luteolin attenuates RA-associated chronic pain by targeting the LDHA/H3K9la/NFATC2 axis to suppress Th17 cell differentiation and central infiltration
Journal: Journal Of Pharmaceutical Analysis
DOI: 10.1016/j.jpha.2025.101373
IF: 8.9
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jun
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An antibacterial, antioxidant and hemostatic hydrogel accelerates infectious wound healing
Journal: Journal Of Nanobiotechnology
DOI: 10.1186/s12951-025-03148-w
IF: 12.6
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
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An antibacterial, antioxidant and hemostatic hydrogel accelerates infectious wound healing
Journal: Journal Of Nanobiotechnology
DOI:
IF: 10.6
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
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Recombinant human collagen XVII promotes skin repair and regeneration by upregulating Lgr6 signaling pathway
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.149585
IF: 8.5
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Dec
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(−)-Epicatechin gallate prevented atherosclerosis by reducing abnormal proliferation of VSMCs and oxidative stress of AML 12 cells
Journal: Cellular Signalling
DOI:
IF: 4.4
Application: ELISA
Reactivity: Mouse
Publish date: 2024 Jul