Mouse TSLP enzyme-linked immunoassay kit
Catalog# EM0081
Mouse TSLP enzyme-linked immunoassay kit
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ELISA
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Mouse
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46.88~3000 pg/ml
Overview
Product Name
Mouse TSLP enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.44 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0260 | 0.0211 | 0.0236 | |
| 46.88 | 0.0730 | 0.0807 | 0.0769 | 0.0533 |
| 93.75 | 0.1270 | 0.1218 | 0.1244 | 0.1009 |
| 187.50 | 0.2381 | 0.2263 | 0.2322 | 0.2087 |
| 375.00 | 0.4863 | 0.4643 | 0.4753 | 0.4518 |
| 750.00 | 0.8973 | 0.8371 | 0.8672 | 0.8437 |
| 1500.00 | 1.6190 | 1.6040 | 1.6115 | 1.5880 |
| 3000.00 | 2.8250 | 2.9140 | 2.8695 | 2.8460 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 76.6 | 357.2 | 987.1 | 71.5 | 365.3 | 1014.8 |
| Standard Deviation | 3.2 | 14.8 | 53.5 | 4.9 | 14.6 | 36.3 |
| Coefficient of Variation (%) | 6.5 | 6.3 | 6.9 | 6.9 | 4.0 | 3.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse TSLP into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 117% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-13.08 | 6.25 | 13.08 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of TSLP in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃