Mouse Angiotensinogen/AGT enzyme-linked immunoassay kit(one step)
Catalog# EMY0055
Mouse Angiotensinogen/AGT enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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4.10~1000 ng/ml
Overview
Product Name
Mouse Angiotensinogen/AGT enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.18 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0070 | 0.0058 | 0.0064 | |
| 4.10 | 0.0238 | 0.0241 | 0.0240 | 0.0176 |
| 10.24 | 0.0523 | 0.0545 | 0.0534 | 0.0470 |
| 25.60 | 0.1480 | 0.1288 | 0.1384 | 0.1320 |
| 64.00 | 0.3872 | 0.3845 | 0.3859 | 0.3795 |
| 160.00 | 0.9081 | 1.0140 | 0.9611 | 0.9547 |
| 400.00 | 2.2850 | 2.0880 | 2.1865 | 2.1801 |
| 1000.00 | 3.7610 | 3.6150 | 3.6880 | 3.6816 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 21.9 | 108.5 | 346.2 | 18.7 | 86.6 | 261.0 |
| Standard Deviation | 1.2 | 5.4 | 16.0 | 1.2 | 3.2 | 14.9 |
| Coefficient of Variation (%) | 5.7 | 5.0 | 4.6 | 6.6 | 3.7 | 5.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse Angiotensinogen/AGT into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 115% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.63-11.41 | 100 | 9.86 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Angiotensinogen/AGT in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃