Mouse IL-22 enzyme-linked immunoassay kit
Catalog# EM0021
Mouse IL-22 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
13.72~10000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Mouse IL-22 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
11.2 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0691 | 0.0692 | 0.0692 | |
| 13.72 | 0.0762 | 0.0771 | 0.0767 | 0.0075 |
| 41.15 | 0.0931 | 0.0961 | 0.0946 | 0.0254 |
| 123.46 | 0.1505 | 0.1532 | 0.1519 | 0.0827 |
| 370.37 | 0.3031 | 0.3022 | 0.3027 | 0.2335 |
| 1111.11 | 0.7051 | 0.6984 | 0.7018 | 0.6326 |
| 3333.33 | 1.7461 | 1.7731 | 1.7596 | 1.6904 |
| 10000.00 | 3.6061 | 3.5883 | 3.5972 | 3.5280 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 145.2 | 826.5 | 3063.2 | 135.4 | 867.1 | 2965.3 |
| Standard Deviation | 8.1 | 46.4 | 8.1 | 7.2 | 49.7 | 93.6 |
| Coefficient of Variation (%) | 5.6 | 5.6 | 4.8 | 5.3 | 5.7 | 3.2 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 IL-22，未加小鼠IL-22 的血清作为本底，计算回收率。回收率的范围从 86 % 至 104 %，平均回收率为 97 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-679.5 | 75 | 108.1 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Cytokine Zcyto18
IL 10 related T cell derived inducible factor
IL 21
IL 22
IL D110
IL TIF
IL-10-related T-cell-derived-inducible factor
IL-22
IL-TIF
IL21
ExpandFunction
IL-22（白细胞介素-22）是一种细胞因子，可在炎症期间诱导活性氧IL-6，IL-10和TNF-α的产生以及中性粒细胞浸润。它还支持上皮屏障的完整性，并在伤口愈合过程中诱导上皮细胞增殖。IL-22 通过由 IL-22 R 和 IL-10 R β 组成的受体复合物发出信号。IL-10 R β 是 IL-10、IL-26、IL-28 和 IL-29 受体复合物的共享成分。IL-22还与IL-22BP结合，阻断IL-22与IL-22 R的相互作用。