Mouse CXCL10/IP-10/CRG-2 enzyme-linked immunoassay kit
Catalog# EM0014
Mouse CXCL10/IP-10/CRG-2 enzyme-linked immunoassay kit
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ELISA
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Mouse
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312.5~20000 pg/ml
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unconjugated
Overview
Product Name
Mouse CXCL10/IP-10/CRG-2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
213.35 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0714 | 0.0721 | 0.0718 | |
| 625.00 | 0.1255 | 0.1243 | 0.1249 | 0.053 |
| 1250.00 | 0.3262 | 0.3452 | 0.3357 | 0.264 |
| 2500.00 | 0.7720 | 0.7891 | 0.7806 | 0.709 |
| 5000.00 | 1.7940 | 1.6820 | 1.7380 | 1.666 |
| 10000.00 | 2.8290 | 2.8190 | 2.8240 | 2.752 |
| 20000.00 | 4.1393 | 3.9812 | 4.0603 | 3.989 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 1023.2 | 3998.7 | 8588.8 | 1179.3 | 4202.1 | 8465.1 |
| Standard Deviation | 62.1 | 188.3 | 664.3 | 79.0 | 111.8 | 378.3 |
| Coefficient of Variation (%) | 6.1 | 4.7 | 7.7 | 6.7 | 2.7 | 4.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL10 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 114% with an overall mean recovery of 97%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-437.15 | 21 | 279.03 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL10 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Mouse | See 1 publications below |
Target
UNIPROT
Alias
C-X-C motif chemokine 10
10 kDa interferon gamma-induced protein (Gamma-IP10
IP-10)
C7
Interferon-gamma induced protein CRG-2
Small-inducible cytokine B10
Cxcl10
Crg2
Ifi10
Inp10
ExpandFunction
Citation
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Pathogen-derived glyoxylate inhibits Tet2 DNA dioxygenase to facilitate bacterial persister formation
Journal: Cell Metabolism
DOI: 10.1016/j.cmet.2025.01.019
IF: 27.7
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Mar