Mouse/Rat Prealbumin/Transthyretin enzyme-linked immunoassay kit(one step)
Catalog# EMY0066
Mouse/Rat Prealbumin/Transthyretin enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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1.37~1000 ng/ml
Overview
Product Name
Mouse/Rat Prealbumin/Transthyretin enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.24 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0089 | 0.0094 | 0.0092 | |
| 1.37 | 0.0149 | 0.0151 | 0.0150 | 0.0059 |
| 4.12 | 0.0272 | 0.0294 | 0.0283 | 0.0192 |
| 12.35 | 0.0699 | 0.0702 | 0.0701 | 0.0609 |
| 37.04 | 0.2013 | 0.1948 | 0.1981 | 0.1889 |
| 111.11 | 0.5377 | 0.5528 | 0.5453 | 0.5361 |
| 333.33 | 1.3140 | 1.2280 | 1.2710 | 1.2619 |
| 1000.00 | 2.6870 | 2.7300 | 2.7085 | 2.6994 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 15.0 | 83.1 | 302.5 | 17.5 | 89.7 | 299.9 |
| Standard Deviation | 0.5 | 3.2 | 12.9 | 0.6 | 3.9 | 10.3 |
| Coefficient of Variation (%) | 3.3 | 3.8 | 4.3 | 3.7 | 4.4 | 3.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat Prealbumin/Transthyretin into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 120% with an overall mean recovery of 97%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 246.57-496.44 | 100 | 376.37 |
| Rat Serum | 30 | 109.17-395.07 | 100 | 244.00 |
Serum/Plasma – Thirty samples from apparently healthy mice/rats were evaluated for the presence of Prealbumin/Transthyretin in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃