Mouse/Rat P-Cadherin/Cadherin-3/CDH3 enzyme-linked immunoassay kit(one step)
Catalog# EMY0084
Mouse/Rat P-Cadherin/Cadherin-3/CDH3 enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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0.69 ~500 ng/ml
Overview
Product Name
Mouse/Rat P-Cadherin/Cadherin-3/CDH3 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.09ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0066 | 0.0066 | 0.0066 | |
| 0.69 | 0.0116 | 0.0125 | 0.0121 | 0.0055 |
| 2.06 | 0.0239 | 0.0247 | 0.0243 | 0.0177 |
| 6.17 | 0.0655 | 0.0662 | 0.0659 | 0.0593 |
| 18.52 | 0.1882 | 0.1868 | 0.1875 | 0.1809 |
| 55.56 | 0.5271 | 0.5229 | 0.5250 | 0.5184 |
| 166.67 | 1.4330 | 1.4280 | 1.4305 | 1.4239 |
| 500.00 | 3.2280 | 3.1870 | 3.2075 | 3.2009 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 7.4 | 39.2 | 130.1 | 8.1 | 41.9 | 128.7 |
| Standard Deviation | 0.4 | 2.5 | 7.5 | 0.5 | 2.2 | 6.1 |
| Coefficient of Variation (%) | 5.7 | 6.3 | 5.7 | 6.8 | 5.2 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat P-Cadherin/Cadherin-3/CDH3 into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 119% with an overall mean recovery of 112%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 2.02-16.27 | 100 | 10.59 |
| Rat Serum | 30 | 3.60-58.20 | 100 | 28.97 |
Serum/Plasma – Thirty samples from apparently healthy mice and thirty samples from apparently healthy rats were evaluated for the presence of mouse/rat P-Cadherin/Cadherin-3/CDH3 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
Alias
Cadherin-3
Placental cadherin (P-cadherin)
Cdh3
Cdhp