Mouse/Rat HGF enzyme-linked immunoassay kit
Catalog# EM0072
Mouse/Rat HGF enzyme-linked immunoassay kit
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ELISA
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Mouse
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Rat
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468.75 ~30000 pg/ml
Overview
Product Name
Mouse/Rat HGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
154.72 pg/ml (50 μl)；622.09 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0110 | 0.0114 | 0.0112 | |
| 468.75 | 0.0184 | 0.0184 | 0.0184 | 0.0072 |
| 937.50 | 0.0326 | 0.0334 | 0.0330 | 0.0218 |
| 1875.00 | 0.0946 | 0.0918 | 0.0932 | 0.0820 |
| 3750.00 | 0.2929 | 0.2983 | 0.2956 | 0.2844 |
| 7500.00 | 0.8757 | 0.8497 | 0.8627 | 0.8515 |
| 15000.00 | 2.0480 | 1.9940 | 2.0210 | 2.0098 |
| 30000.00 | 3.4110 | 3.5690 | 3.4900 | 3.4788 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 666.3 | 3125.1 | 9776.9 | 662.5 | 3043.2 | 9185.5 |
| Standard Deviation | 28.6 | 31.0 | 363.4 | 23.6 | 34.8 | 440.8 |
| Coefficient of Variation (%) | 4.8 | 2.0 | 3.4 | 3.6 | 1.1 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat HGF into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 115% with an overall mean recovery of 88%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 2.24-20.62 | 100 | 5.52 |
| Rat Serum | 30 | 12.11-51.87 | 100 | 26.58 |
Serum/Plasma – Thirty samples from apparently healthy mice and thirty samples from apparently healthy rats were evaluated for the presence of mouse/rat HGF in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃