Mouse/Rat GDF-15 enzyme-linked immunoassay kit
Catalog# EM0058
Mouse/Rat GDF-15 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
4.10~1000 pg/ml
Overview
Product Name
Mouse/Rat GDF-15 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.20 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0175 | 0.0162 | 0.0169 | |
| 4.10 | 0.0347 | 0.0336 | 0.0342 | 0.0173 |
| 10.24 | 0.0514 | 0.0499 | 0.0507 | 0.0338 |
| 25.60 | 0.1131 | 0.1059 | 0.1095 | 0.0927 |
| 64.00 | 0.2521 | 0.2470 | 0.2496 | 0.2327 |
| 160.00 | 0.6446 | 0.5856 | 0.6151 | 0.5983 |
| 400.00 | 1.4250 | 1.4270 | 1.4260 | 1.4092 |
| 1000.00 | 2.9220 | 2.9260 | 2.9240 | 2.9072 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 38.8 | 143.9 | 357.6 | 39.0 | 143.8 | 359.6 |
| Standard Deviation | 1.0 | 3.0 | 10.8 | 1.0 | 2.5 | 7.7 |
| Coefficient of Variation (%) | 2.4 | 2.1 | 3.0 | 2.5 | 1.7 | 2.1 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠/大鼠 GDF-15，未加小鼠/大鼠的GDF-15血清作为本底，计算回收率。回收率的范围从 105 % 至 125 %，平均回收率为 129 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.57-33.73 | 100 | 20.84 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
Alias
GDF 15
GDF-15
Gdf15
GDF15_HUMAN
Growth differentiation factor 15
Growth/differentiation factor 15
Macrophage inhibitory cytokine 1
MIC 1
Mic-1
MIC1
ExpandFunction
GDF-15（生长分化因子15）是一种由两个分子组成的TGF-β超家族细胞因子，在胎盘和大脑中含量尤为丰富。它能够促进神经细胞的存活，同时调节神经肽Y及前阿片黑皮质素的水平，从而抑制食欲。此外，GDF-15还能通过抑制血小板活化来保护心脏，预防动脉粥样硬化的发生，促进心肌梗死后的恢复，并调节血管新生过程。同时，它还能抑制软骨的形成，以及LPS刺激下巨噬细胞产生的TNF-α。