Mouse/Rat Galectin-1/LGALS 1 enzyme-linked immunoassay kit
Catalog# EM0088
Mouse/Rat Galectin-1/LGALS 1 enzyme-linked immunoassay kit
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ELISA
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Mouse
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40.96 ~10000 pg/ml
Overview
Product Name
Mouse/Rat Galectin-1/LGALS 1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
10.44pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0389 | 0.0358 | 0.0374 | |
| 40.96 | 0.0528 | 0.0517 | 0.0523 | 0.0149 |
| 102.40 | 0.0805 | 0.0753 | 0.0779 | 0.0406 |
| 256.00 | 0.1469 | 0.1381 | 0.1425 | 0.1052 |
| 640.00 | 0.3043 | 0.3025 | 0.3034 | 0.2661 |
| 1600.00 | 0.6994 | 0.7174 | 0.7084 | 0.6711 |
| 4000.00 | 1.6320 | 1.5880 | 1.6100 | 1.5727 |
| 10000.00 | 3.4380 | 3.4400 | 3.4390 | 3.4017 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 212.8 | 1018.4 | 3111.5 | 225.4 | 1101.1 | 3275.6 |
| Standard Deviation | 10.1 | 50.9 | 226.1 | 16.3 | 39.7 | 60.1 |
| Coefficient of Variation (%) | 4.8 | 5.0 | 7.3 | 7.2 | 3.6 | 1.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat Galectin-1/LGALS1 into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 107%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 8.79-28.10 | 100 | 19.50 |
| Rat Serum | 30 | 11.93-73.91 | 100 | 28.40 |
Serum/Plasma – Thirty samples from apparently healthy mice and thirty samples from apparently healthy rats were evaluated for the presence of Galectin-1/LGALS1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
Alias
Galectin-1
Gal-1
14 kDa lectin
Beta-galactoside-binding lectin L-14-I
Galaptin
Lactose-binding lectin 1
Lectin galactoside-binding soluble 1
S-Lac lectin 1
Lgals1
Gbp