Mouse/Rat CD38 enzyme-linked immunoassay kit
Catalog# EM0092
Mouse/Rat CD38 enzyme-linked immunoassay kit
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ELISA
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Mouse
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12.29 ~3000 pg/ml
Overview
Product Name
Mouse/Rat CD38 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.46 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0559 | 0.0568 | 0.0564 | |
| 12.29 | 0.0864 | 0.0851 | 0.0858 | 0.0294 |
| 30.72 | 0.1195 | 0.1260 | 0.1228 | 0.0664 |
| 76.80 | 0.2224 | 0.2139 | 0.2182 | 0.1618 |
| 192.00 | 0.4462 | 0.4426 | 0.4444 | 0.3881 |
| 480.00 | 0.9767 | 0.9764 | 0.9766 | 0.9202 |
| 1200.00 | 2.0930 | 2.0860 | 2.0895 | 2.0332 |
| 3000.00 | 3.7640 | 3.5980 | 3.6810 | 3.6247 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 54.4 | 279.9 | 864.0 | 57.2 | 300.5 | 884.0 |
| Standard Deviation | 2.2 | 14.4 | 45.3 | 2.4 | 16.3 | 24.5 |
| Coefficient of Variation (%) | 4.0 | 5.2 | 5.2 | 4.3 | 5.4 | 2.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat CD38 into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 97%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 115.90-360.35 | 100 | 297.25 |
| Rat Serum | 30 | 8.68-53.49 | 100 | 21.16 |
Serum/Plasma – Thirty samples from apparently healthy mice and thirty samples from apparently healthy rats were evaluated for the presence of CD38 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
Alias
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
EC:3.2.2
EC:3.2.2.6
2'-phospho-ADP-ribosyl cyclase
2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase
2'-phospho-cyclic-ADP-ribose transferase
ADP-ribosyl cyclase 1 (ADPRC 1)
Cyclic ADP-ribose hydrolase 1 (cADPR hydrolase 1)
I-19
NIM-R5 antigen
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