Mouse/Rat VEGFR3/FLT4 enzyme-linked immunoassay kit(one step)
Catalog# EMY0074
Mouse/Rat VEGFR3/FLT4 enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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Rat
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7.81~500 ng/ml
Overview
Product Name
Mouse/Rat VEGFR3/FLT4 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.18 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0064 | 0.0066 | 0.0066 | |
| 7.81 | 0.0530 | 0.0511 | 0.0521 | 0.0455 |
| 15.63 | 0.1109 | 0.1045 | 0.1077 | 0.1011 |
| 31.25 | 0.2141 | 0.2223 | 0.2182 | 0.2116 |
| 62.50 | 0.4542 | 0.4671 | 0.4607 | 0.4541 |
| 125.00 | 0.9015 | 0.9226 | 0.9121 | 0.9055 |
| 250.00 | 1.8140 | 1.8360 | 1.8250 | 1.8184 |
| 500.00 | 3.1490 | 3.1270 | 3.1380 | 3.1314 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 10.0 | 47.4 | 152.3 | 10.4 | 48.9 | 160.6 |
| Standard Deviation | 0.5 | 1.7 | 5.2 | 0.5 | 1.6 | 7.3 |
| Coefficient of Variation (%) | 4.8 | 3.7 | 3.4 | 4.3 | 3.2 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat VEGFR3/FLT4 into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 112% with an overall mean recovery of 101%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 94.52-190.88 | 100 | 159.39 |
| Rat Serum | 30 | 90.56-139.67 | 100 | 111.39 |
Serum/Plasma – Thirty samples from apparently healthy mice and thirty samples from apparently healthy rats were evaluated for the presence of mouse/rat VEGFR3/FLT4 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃