Mouse/Rat Von Willebrand Factor/vWF enzyme-linked immunoassay kit（one step）
Catalog# EMY0079
Mouse/Rat Von Willebrand Factor/vWF enzyme-linked immunoassay kit（one step）
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ELISA
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Mouse
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15.63~1000 pg/ml
Overview
Product Name
Mouse/Rat Von Willebrand Factor/vWF enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.07 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0087 | 0.0089 | 0.0088 | |
| 15.63 | 0.0551 | 0.0542 | 0.0547 | 0.0459 |
| 31.25 | 0.1052 | 0.1039 | 0.1046 | 0.0958 |
| 62.50 | 0.1997 | 0.2040 | 0.2019 | 0.1931 |
| 125.00 | 0.4026 | 0.4122 | 0.4074 | 0.3986 |
| 250.00 | 0.7606 | 0.8061 | 0.7834 | 0.7746 |
| 500.00 | 1.4480 | 1.4910 | 1.4695 | 1.4607 |
| 1000.00 | 2.5890 | 2.5310 | 2.5600 | 2.5512 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 20.7 | 97.2 | 277.5 | 19.5 | 93.1 | 274.8 |
| Standard Deviation | 0.9 | 4.8 | 10.3 | 0.9 | 4.2 | 12.6 |
| Coefficient of Variation (%) | 4.6 | 5.0 | 3.7 | 4.6 | 4.5 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat Von Willebrand Factor/vWF into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 58.63-152.00 | 100 | 80.70 |
| Rat Serum | 30 | 179.60-432.13 | 100 | 268.37 |
Serum/Plasma – Thirty samples from apparently healthy mice and thirty samples from apparently healthy rats were evaluated for the presence of mouse/rat Von Willebrand Factor/vWF in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃