Mouse FGF-21 enzyme-linked immunoassay kit
Catalog# EM0053
Mouse FGF-21 enzyme-linked immunoassay kit
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ELISA
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Mouse
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8.19~2000 pg/ml
Overview
Product Name
Mouse FGF-21 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.46 pg/ml（10 μl）
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0171 | 0.0175 | 0.0173 | |
| 8.19 | 0.0256 | 0.0269 | 0.0263 | 0.0090 |
| 20.48 | 0.0374 | 0.0406 | 0.0390 | 0.0217 |
| 51.20 | 0.0727 | 0.0785 | 0.0756 | 0.0583 |
| 128.00 | 0.1705 | 0.1781 | 0.1743 | 0.1570 |
| 320.00 | 0.3888 | 0.4356 | 0.4122 | 0.3949 |
| 800.00 | 0.9714 | 1.0840 | 1.0277 | 1.0104 |
| 2000.00 | 2.8700 | 2.5260 | 2.6980 | 2.6807 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 55.4 | 247.5 | 704.8 | 44.6 | 210.5 | 599.7 |
| Standard Deviation | 2.7 | 12.9 | 40.7 | 1.3 | 9.7 | 13.3 |
| Coefficient of Variation (%) | 4.8 | 5.2 | 5.8 | 2.9 | 4.6 | 2.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse FGF-21 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 120% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.29-921.03 | 100 | 196.81 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of FGF-21 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
FGF 21
FGF-21
Fgf21
FGF21_HUMAN
FGFL
Fibroblast growth factor 21
PRO10196
UNQ3115