Mouse NCR1/NKp46 enzyme-linked immunoassay kit
Catalog# EM0080
Mouse NCR1/NKp46 enzyme-linked immunoassay kit
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ELISA
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Mouse
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12.29~3000 pg/ml
Overview
Product Name
Mouse NCR1/NKp46 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.67 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0208 | 0.0221 | 0.0215 | |
| 12.29 | 0.0381 | 0.0390 | 0.0386 | 0.0171 |
| 30.72 | 0.0657 | 0.0671 | 0.0664 | 0.0450 |
| 76.80 | 0.1320 | 0.1259 | 0.1290 | 0.1075 |
| 192.00 | 0.2958 | 0.2898 | 0.2928 | 0.2714 |
| 480.00 | 0.6617 | 0.6678 | 0.6648 | 0.6433 |
| 1200.00 | 1.5220 | 1.5940 | 1.5580 | 1.5366 |
| 3000.00 | 3.0500 | 3.0740 | 3.0620 | 3.0406 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 57.4 | 274.0 | 868.2 | 59.0 | 274.3 | 822.0 |
| Standard Deviation | 2.6 | 12.5 | 35.3 | 2.4 | 8.0 | 33.1 |
| Coefficient of Variation (%) | 4.5 | 4.6 | 4.1 | 4.1 | 2.9 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse NCR1/NKp46 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 104% with an overall mean recovery of 94%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 6.35-90.69 | 100 | 20.14 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of NCR1/NKp46 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃