Mouse alpha 1 antitrypsin enzyme-linked immunoassay kit（one step）
Catalog# EMY0052
Mouse alpha 1 antitrypsin enzyme-linked immunoassay kit（one step）
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ELISA
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Mouse
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13.72~10000 pg/ml
Overview
Product Name
Mouse alpha 1 antitrypsin enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
1.57 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0085 | 0.0087 | 0.0086 | |
| 13.72 | 0.0168 | 0.0182 | 0.0175 | 0.0089 |
| 41.15 | 0.0345 | 0.0348 | 0.0347 | 0.0261 |
| 123.46 | 0.0903 | 0.0873 | 0.0888 | 0.0802 |
| 370.37 | 0.2459 | 0.2427 | 0.2443 | 0.2357 |
| 1111.11 | 0.6791 | 0.6760 | 0.6776 | 0.6690 |
| 3333.33 | 1.7800 | 1.7870 | 1.7835 | 1.7749 |
| 10000.00 | 3.7750 | 3.8150 | 3.7950 | 3.7864 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 203.5 | 1135.4 | 4091.5 | 165.0 | 911.8 | 3156.6 |
| Standard Deviation | 15.0 | 70.0 | 273.9 | 8.7 | 57.5 | 117.3 |
| Coefficient of Variation (%) | 7.4 | 6.2 | 6.7 | 5.3 | 6.3 | 3.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse alpha 1 antitrypsin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 210.39-335.68 | 100 | 288.48 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of alpha 1 antitrypsin in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
A1A
A1AT
A1AT_HUMAN
AAT
Alpha 1 antiproteinase
Alpha 1 antitrypsin
Alpha 1 antitrypsin null
Alpha 1 protease inhibitor
Alpha-1 protease inhibitor
Alpha-1-antiproteinase
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