Mouse alpha 1 antitrypsin enzyme-linked immunoassay kit（one step）
Catalog# EMY0052
Mouse alpha 1 antitrypsin enzyme-linked immunoassay kit（one step）
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ELISA
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Mouse
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13.72~10000 pg/ml
Overview
Product Name
Mouse alpha 1 antitrypsin enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
1.57 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0085 | 0.0087 | 0.0086 | |
| 13.72 | 0.0168 | 0.0182 | 0.0175 | 0.0089 |
| 41.15 | 0.0345 | 0.0348 | 0.0347 | 0.0261 |
| 123.46 | 0.0903 | 0.0873 | 0.0888 | 0.0802 |
| 370.37 | 0.2459 | 0.2427 | 0.2443 | 0.2357 |
| 1111.11 | 0.6791 | 0.6760 | 0.6776 | 0.6690 |
| 3333.33 | 1.7800 | 1.7870 | 1.7835 | 1.7749 |
| 10000.00 | 3.7750 | 3.8150 | 3.7950 | 3.7864 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 203.5 | 1135.4 | 4091.5 | 165.0 | 911.8 | 3156.6 |
| Standard Deviation | 15.0 | 70.0 | 273.9 | 8.7 | 57.5 | 117.3 |
| Coefficient of Variation (%) | 7.4 | 6.2 | 6.7 | 5.3 | 6.3 | 3.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 alpha 1 antitrypsin，未加小鼠 alpha 1 antitrypsin 的血清作为本底，计算回收率。回收率的范围从 90 % 至 120 %，平均回收率为 102 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 210.39-335.68 | 100 | 288.48 |
应用本试剂盒，检测 30 只健康小鼠的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
A1A
A1AT
A1AT_HUMAN
AAT
Alpha 1 antiproteinase
Alpha 1 antitrypsin
Alpha 1 antitrypsin null
Alpha 1 protease inhibitor
Alpha-1 protease inhibitor
Alpha-1-antiproteinase
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