Mouse IL-3 enzyme-linked immunoassay kit
Catalog# EM0048
Mouse IL-3 enzyme-linked immunoassay kit
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ELISA
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Mouse
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31.25~2000 pg/ml
Overview
Product Name
Mouse IL-3 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.94 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0303 | 0.0328 | 0.0316 | |
| 31.25 | 0.1063 | 0.1099 | 0.1081 | 0.0766 |
| 62.50 | 0.1745 | 0.1872 | 0.1809 | 0.1493 |
| 125.00 | 0.3338 | 0.3315 | 0.3327 | 0.3011 |
| 250.00 | 0.5987 | 0.6882 | 0.6435 | 0.6119 |
| 500.00 | 1.1760 | 1.2480 | 1.2120 | 1.1805 |
| 1000.00 | 2.1960 | 2.2850 | 2.2405 | 2.2090 |
| 2000.00 | 3.7180 | 3.8870 | 3.8025 | 3.7710 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 48.7 | 226.6 | 675.3 | 51.6 | 248.0 | 692.7 |
| Standard Deviation | 1.4 | 9.1 | 18.4 | 2.3 | 8.3 | 32.7 |
| Coefficient of Variation (%) | 2.9 | 4.0 | 2.7 | 4.4 | 3.4 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-3 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 104% to 118% with an overall mean recovery of 112%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-7.02 | 60 | 3.12 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-3 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Colony stimulating factor multiple
Hematopoietic growth factor
IL 3
IL-3
IL3
IL3_HUMAN
Interleukin 3 (colony stimulating factor
multiple)
Interleukin 3
Interleukin-3
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