Mouse CD200/OX2 enzyme-linked immunoassay kit
Catalog# EM0091
Mouse CD200/OX2 enzyme-linked immunoassay kit
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ELISA
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Mouse
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15.63 ~1000 pg/ml
Overview
Product Name
Mouse CD200/OX2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.13 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0113 | 0.0106 | 0.0110 | |
| 15.63 | 0.0972 | 0.0980 | 0.0976 | 0.0867 |
| 31.25 | 0.1881 | 0.1881 | 0.1881 | 0.1772 |
| 62.50 | 0.3766 | 0.3581 | 0.3674 | 0.3564 |
| 125.00 | 0.7547 | 0.7285 | 0.7416 | 0.7307 |
| 250.00 | 1.4150 | 1.4170 | 1.4160 | 1.4051 |
| 500.00 | 2.5000 | 2.5770 | 2.5385 | 2.5276 |
| 1000.00 | 3.7960 | 3.7070 | 3.7515 | 3.7406 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 21.0 | 98.7 | 286.6 | 22.4 | 103.0 | 286.6 |
| Standard Deviation | 1.0 | 4.1 | 17.1 | 0.8 | 4.7 | 8.1 |
| Coefficient of Variation (%) | 4.6 | 4.1 | 6.0 | 3.8 | 4.6 | 2.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CD200/OX2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 117% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2990.65-5523.47 | 100 | 4314.28 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of mouse CD200/OX2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Antigen identified by monoclonal antibody MRC OX 2 antibody
CD200 antibody
CD200 antigen antibody
CD200 molecule antibody
MOX1 antibody
MOX2 antibody
MRC antibody
MRC OX 2 antigen antibody
My033 antibody
OX 2 antibody
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