Mouse IL-33 enzyme-linked immunoassay kit
Catalog# EM0033
Mouse IL-33 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
6.86~5000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Mouse IL-33 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.33 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0176 | 0.0174 | 0.0175 | |
| 6.86 | 0.0225 | 0.0230 | 0.0228 | 0.0053 |
| 20.58 | 0.0342 | 0.0350 | 0.0346 | 0.0171 |
| 61.73 | 0.0675 | 0.0710 | 0.0693 | 0.0518 |
| 185.19 | 0.1737 | 0.1767 | 0.1752 | 0.1577 |
| 555.56 | 0.5058 | 0.5463 | 0.5261 | 0.5086 |
| 1666.67 | 1.3030 | 1.4530 | 1.3780 | 1.3605 |
| 5000.00 | 3.0570 | 3.1460 | 3.1015 | 3.0840 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 117.2 | 525.0 | 1698.4 | 89.6 | 465.7 | 1468.5 |
| Standard Deviation | 5.8 | 33.7 | 75.0 | 2.8 | 22.1 | 69,2 |
| Coefficient of Variation (%) | 5.0 | 6.4 | 4.4 | 3.2 | 4.7 | 4.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠IL-33，未加小鼠IL-33的血清作为本底，计算回收率。回收率的范围从 84 % 至 107 %，平均回收率为 94 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.55 | 53 | 0.53 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C9orf26
CHROMOSOME 9 OPEN READING FRAME 26
DKFZp586H0523
DVS27
DVS27 related protein
IL 1F11
IL 33
IL-1F11
IL-33
IL1F11
ExpandFunction
IL-33（白细胞介素-33）从物理损伤或坏死细胞中释放出来。它在炎症部位触发Th2偏向的免疫细胞活化以及调节性T细胞和M2巨噬细胞扩增。它还诱导血管生成，促进肿瘤细胞迁移和侵袭，限制心肌细胞肥大，并限制动脉粥样硬化斑块的发展。IL-33通过由ST2和IL-1 RAcP组成的受体复合物发出信号。IL-1 RAcP 还与 IL-1 RI、IL-1 RII、IL-1 R6 和 SCF R/c-kit 相关联。ST2和IL-1 RAcP的可溶性亚型作为调节IL-33生物活性的诱饵受体起作用。