Mouse IL-13 enzyme-linked immunoassay kit
Catalog# EM0007
Mouse IL-13 enzyme-linked immunoassay kit
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ELISA
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Mouse
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4.12~3000 pg/ml
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unconjugated
Overview
Product Name
Mouse IL-13 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.37 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0252 | 0.0263 | 0.0258 | |
| 4.12 | 0.0451 | 0.0451 | 0.0451 | 0.0194 |
| 12.35 | 0.0781 | 0.0772 | 0.0777 | 0.0519 |
| 37.04 | 0.1694 | 0.1712 | 0.1703 | 0.1446 |
| 111.11 | 0.4072 | 0.3993 | 0.4033 | 0.3775 |
| 333.33 | 0.9812 | 0.9731 | 0.9772 | 0.9514 |
| 1000 | 2.3813 | 2.3685 | 2.3749 | 2.3492 |
| 3000 | 4.4451 | 4.5012 | 4.4732 | 4.4474 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 62.3 | 312.1 | 923.2 | 68.9 | 323.2 | 916.4 |
| Standard Deviation | 3.1 | 20.0 | 60.9 | 4.6 | 17.5 | 48.6 |
| Coefficient of Variation (%) | 4.9 | 6.4 | 6.6 | 6.6 | 5.4 | 5.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-13 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 115% with an overall mean recovery of 101%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.73-15.97 | 100 | 2.35 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-13 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Mouse | See 1 publications below |
Target
UNIPROT
Alias
Allergic rhinitis
ALRH
BHR 1
BHR1
Bronchial hyperresponsiveness 1 (bronchial asthma)
IL 13
IL-13
Il13
IL13_HUMAN
Interleukin 13
ExpandFunction
Citation
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Bach2 antagonizes leukotriene B4-Ltb4r1-JunB signaling to constrain Tfh13 cell differentiation
Journal: Cell Reports
DOI: 10.1016/j.celrep.2026.117220
IF: 6.9
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Apr