Mouse CRP/C-Reactive Protein enzyme-linked immunoassay kit
Catalog# EM0035
Mouse CRP/C-Reactive Protein enzyme-linked immunoassay kit
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sELISA
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Mouse
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15.63~1000 ng/ml
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unconjugated
Overview
Product Name
Mouse CRP/C-Reactive Protein enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.38 ng/ml (50 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0323 | 0.0317 | 0.0320 | |
| 15.63 | 0.1721 | 0.1720 | 0.1721 | 0.1401 |
| 31.25 | 0.2981 | 0.2878 | 0.2930 | 0.2610 |
| 62.50 | 0.5420 | 0.5600 | 0.5510 | 0.5190 |
| 125.00 | 1.0590 | 1.0680 | 1.0635 | 1.0315 |
| 250.00 | 1.9160 | 1.8320 | 1.8740 | 1.8420 |
| 500.00 | 3.0340 | 3.0100 | 3.0220 | 2.9900 |
| 1000.00 | 4.0137 | 3.9687 | 3.9912 | 3.9592 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 20.89 | 97.52 | 317.93 | 21.39 | 123.08 | 333.47 |
| Standard Deviation | 1.74 | 8.01 | 27.55 | 1.48 | 8.87 | 14.64 |
| Coefficient of Variation (%) | 8.4 | 8.2 | 8.7 | 6.9 | 7.2 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CRP into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 115% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.86-10.59 | 100 | 5.62 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CRP in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Pentraxin 1, short
C reactive protein
C reactive protein pentraxin related
C-reactive protein(1-205)
CRP
CRP_HUMAN
MGC88244
Pentraxin 1
PTX 1
PTX1