Mouse CRP/C-Reactive Protein enzyme-linked immunoassay kit
Catalog# EM0035
Mouse CRP/C-Reactive Protein enzyme-linked immunoassay kit
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ELISA
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Mouse
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0.14~100 ng/ml
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unconjugated
Overview
Product Name
Mouse CRP/C-Reactive Protein enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (50 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0134 | 0.0136 | 0.0135 | |
| 0.14 | 0.0183 | 0.0194 | 0.0189 | 0.0054 |
| 0.41 | 0.0288 | 0.0316 | 0.0302 | 0.0167 |
| 1.23 | 0.0649 | 0.0713 | 0.0681 | 0.0546 |
| 3.70 | 0.1768 | 0.1872 | 0.1820 | 0.1685 |
| 11.11 | 0.5001 | 0.5427 | 0.5214 | 0.5079 |
| 33.33 | 1.4370 | 1.5650 | 1.5010 | 1.4875 |
| 100.00 | 3.5510 | 3.6270 | 3.5890 | 3.5755 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.6 | 8.4 | 30.4 | 2.0 | 10.0 | 32.0 |
| Standard Deviation | 0.1 | 0.3 | 1.1 | 0.0 | 0.2 | 0.6 |
| Coefficient of Variation (%) | 3.6 | 3.0 | 3.7 | 1.4 | 1.6 | 1.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CRP into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 103% to 136% with an overall mean recovery of 125%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 25.47-93.93 | 100 | 64.56 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CRP in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Pentraxin 1, short
C reactive protein
C reactive protein pentraxin related
C-reactive protein(1-205)
CRP
CRP_HUMAN
MGC88244
Pentraxin 1
PTX 1
PTX1