Mouse IGFBP6 enzyme-linked immunoassay kit
Catalog# EM0085
Mouse IGFBP6 enzyme-linked immunoassay kit
-
sELISA
-
Mouse
-
156.25 ~10000 pg/ml
Overview
Product Name
Mouse IGFBP6 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
12.47 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0509 | 0.0496 | 0.0503 | |
| 156.25 | 0.1246 | 0.1145 | 0.1196 | 0.0693 |
| 312.50 | 0.1860 | 0.1937 | 0.1899 | 0.1396 |
| 625.00 | 0.3514 | 0.3276 | 0.3395 | 0.2893 |
| 1250.00 | 0.6322 | 0.6298 | 0.6310 | 0.5808 |
| 2500.00 | 1.1910 | 1.2870 | 1.2390 | 1.1888 |
| 5000.00 | 2.3440 | 2.4260 | 2.3850 | 2.3348 |
| 10000.00 | 4.1115 | 4.1452 | 4.1284 | 4.0781 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 199.3 | 1019.4 | 3084.6 | 209.7 | 1048.7 | 3142.2 |
| Standard Deviation | 11.9 | 50.8 | 169.7 | 11.8 | 39.6 | 74.9 |
| Coefficient of Variation (%) | 6.0 | 5.0 | 5.5 | 5.6 | 3.8 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IGFBP6 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 112% with an overall mean recovery of 97%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 20.32-78.80 | 100 | 44.47 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of mouse IGFBP6 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
IBP 6
IBP-6
IBP6
IGF binding protein 6
IGF-binding protein 6
IGFBP 6
IGFBP-6
IGFBP6
Insulin like growth factor binding protein 6
Insulin-like growth factor-binding protein 6