Mouse Complement factor D enzyme-linked immunoassay kit(one step)
Catalog# EMY0083
Mouse Complement factor D enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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0.69 ~500 ng/ml
Overview
Product Name
Mouse Complement factor D enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.09ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0049 | 0.0061 | 0.0055 | |
| 0.69 | 0.0204 | 0.0224 | 0.0214 | 0.0159 |
| 2.06 | 0.0521 | 0.0520 | 0.0521 | 0.0466 |
| 6.17 | 0.1302 | 0.1442 | 0.1372 | 0.1317 |
| 18.52 | 0.4134 | 0.4098 | 0.4116 | 0.4061 |
| 55.56 | 0.9880 | 1.0940 | 1.0410 | 1.0355 |
| 166.67 | 2.3720 | 2.4590 | 2.4155 | 2.4100 |
| 500.00 | 4.1428 | 3.9500 | 4.0464 | 4.0409 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 12.4 | 58.0 | 171.5 | 10.9 | 52.6 | 158.0 |
| Standard Deviation | 0.8 | 4.4 | 13.0 | 0.6 | 3.0 | 10.2 |
| Coefficient of Variation (%) | 6.8 | 7.6 | 7.6 | 5.8 | 5.8 | 6.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse Complement factor D into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 119% with an overall mean recovery of 115%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 13.55-19.91 | 100 | 17 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Mouse Complement factor D in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Adipsin antibody
Adipsin/complement factor D antibody
Adn antibody
C3 convertase activator antibody
CFAD_HUMAN antibody
CFD antibody
Complement factor D antibody
complement factor D preproprotein antibody
D component of complement antibody
DF antibody
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