Mouse GM-CSF/CSF2 enzyme-linked immunoassay kit
Catalog# EM0011
Mouse GM-CSF/CSF2 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
0.69~500 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Mouse GM-CSF/CSF2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.19 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0831 | 0.0801 | 0.0816 | |
| 0.69 | 0.1011 | 0.0991 | 0.1001 | 0.0185 |
| 2.06 | 0.1453 | 0.1384 | 0.1419 | 0.0603 |
| 6.17 | 0.2796 | 0.2752 | 0.2774 | 0.1958 |
| 18.52 | 0.6674 | 0.5825 | 0.6250 | 0.5434 |
| 55.56 | 1.5812 | 1.4493 | 1.5153 | 1.4337 |
| 166.67 | 3.4412 | 3.1762 | 3.3087 | 3.2271 |
| 500.00 | 4.3492 | 4.3873 | 4.3683 | 4.2867 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 7.6 | 29.4 | 162.5 | 8.2 | 32.2 | 145.1 |
| Standard Deviation | 0.5 | 1.5 | 9.8 | 0.6 | 2.0 | 7.3 |
| Coefficient of Variation (%) | 6.0 | 5.1 | 6.0 | 6.9 | 6.3 | 5.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 GM-CSF/CSF2，未加小鼠GM-CSF/CSF2 的血清作为本底，计算回收率。回收率的范围从 81 % 至 103 %，平均回收率为 92 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d | 0 | 0 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Colony stimulating factor 2 (granulocyte-macrophage)
Colony Stimulating Factor 2
Colony stimulating factor
Colony-stimulating factor
CSF 2
CSF
CSF2
CSF2_HUMAN
GM-CSF
GMCSF
ExpandFunction
GM-CSF（粒细胞-巨噬细胞集落刺激因子）诱导单核细胞、中性粒细胞、嗜酸性粒细胞以及髓系和真皮树突状细胞的发育。它还充当中性粒细胞和树突状细胞化学引诱剂。GM-CSF 促进 Th1 和 Th17 细胞介导的自身免疫性炎症以及树突状细胞、小胶质细胞、肺泡巨噬细胞和嗜酸性粒细胞的炎症激活。此外，它与G-CSF合作促进肿瘤细胞增殖和侵袭。GM-CSF通过由GM-CSF R α和共同β链（β c）组成的受体复合物发出信号，其中Syndecan-2作为潜在的共受体。βc亚基由IL-3和IL-5的受体复合物共享。