Mouse IL-17E enzyme-linked immunoassay kit
Catalog# EM0028
Mouse IL-17E enzyme-linked immunoassay kit
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ELISA
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Mouse
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468.75~30000 pg/ml
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unconjugated
Overview
Product Name
Mouse IL-17E enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
12.33 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0362 | 0.0324 | 0.0343 | |
| 468.75 | 0.0733 | 0.0788 | 0.0761 | 0.0540 |
| 937.50 | 0.1251 | 0.1218 | 0.1235 | 0.1014 |
| 1875.00 | 0.2239 | 0.2252 | 0.2246 | 0.2025 |
| 3750.00 | 0.4344 | 0.4357 | 0.4351 | 0.4130 |
| 7500.00 | 0.8770 | 0.8357 | 0.8564 | 0.8343 |
| 15000.00 | 1.8420 | 1.8410 | 1.8415 | 1.8195 |
| 30000.00 | 3.7110 | 3.8330 | 3.7720 | 3.7500 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 573.6 | 2641.9 | 7988.6 | 582.2 | 2756.5 | 8431.0 |
| Standard Deviation | 38.4 | 115.8 | 476.7 | 38.0 | 133.9 | 514,2 |
| Coefficient of Variation (%) | 6.7 | 4.4 | 6.0 | 6.5 | 4.9 | 6.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-17E/IL-25 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 118% with an overall mean recovery of 109%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-427.60 | 75 | 199.53 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-17E/IL-25 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
IL 17E
IL 25
IL-17E
IL-25
IL17e
IL25
IL25_HUMAN
Interleukin 17E
Interleukin 25
Interleukin-17E
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