Mouse CD86 enzyme-linked immunoassay kit
Catalog# EM0046
Mouse CD86 enzyme-linked immunoassay kit
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ELISA
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Mouse
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6.86~5000 pg/ml
Overview
Product Name
Mouse CD86 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.28 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0093 | 0.0087 | 0.0090 | |
| 6.86 | 0.0362 | 0.0331 | 0.0347 | 0.0257 |
| 20.58 | 0.0949 | 0.0995 | 0.0972 | 0.0882 |
| 61.73 | 0.2952 | 0.3140 | 0.3046 | 0.2956 |
| 185.19 | 0.7960 | 0.7331 | 0.7646 | 0.7556 |
| 555.56 | 1.9620 | 2.0140 | 1.9880 | 1.9790 |
| 1666.67 | 3.2560 | 3.1950 | 3.2255 | 3.2165 |
| 5000.00 | 3.8920 | 3.9040 | 3.8980 | 3.8890 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 182.88 | 551.76 | 1826.16 | 159.79 | 469.11 | 1545.97 |
| Standard Deviation | 7.55 | 19.78 | 110.28 | 8.75 | 26.99 | 89.35 |
| Coefficient of Variation (%) | 4.1 | 3.6 | 6.0 | 5.5 | 5.8 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CD86 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 104% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 119.78-359.50 | 100 | 265.68 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CD86 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Activation B7-2 antigen 3
Activation B7-2 antigen
B-lymphocyte activation antigen B7-2 2
B-lymphocyte activation antigen B7-2
B7 2
B7
B7-2
B7.2
B70
B72 antigen
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