Mouse ACE2 enzyme-linked immunoassay kit
Catalog# EM0089
Mouse ACE2 enzyme-linked immunoassay kit
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sELISA
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Mouse
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122.88~30000 pg/ml
Overview
Product Name
Mouse ACE2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
10.62pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0248 | 0.0241 | 0.0245 | |
| 122.88 | 0.0643 | 0.0632 | 0.0638 | 0.0393 |
| 307.20 | 0.1185 | 0.1182 | 0.1184 | 0.0939 |
| 768.00 | 0.2504 | 0.2530 | 0.2517 | 0.2273 |
| 1920.00 | 0.5608 | 0.5353 | 0.5481 | 0.5236 |
| 4800.00 | 1.1930 | 1.1880 | 1.1905 | 1.1661 |
| 12000.00 | 2.1010 | 2.4190 | 2.2600 | 2.2356 |
| 30000.00 | 3.7010 | 3.9573 | 3.8292 | 3.8047 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 552.9 | 2897.4 | 9193.9 | 574.7 | 2830.5 | 8614.0 |
| Standard Deviation | 21.0 | 98.8 | 299.4 | 27.5 | 106.7 | 268.0 |
| Coefficient of Variation (%) | 3.8 | 3.4 | 3.3 | 4.8 | 3.8 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse ACE2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 121% with an overall mean recovery of 113%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 223.73-1032.10 | 100 | 677.19 |
Serum/Plasma– Thirty samples from apparently healthy mice serum were evaluated for the presence of Mouse ACE2 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
ACE 2
ACE related carboxypeptidase
ACE-related carboxypeptidase
ACE2
ACEH
Angiotensin converting enzyme 2
Angiotensin converting enzyme homolog
Angiotensin converting enzyme like protein
Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2
Angiotensin I converting enzyme 2
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