Mouse LIF enzyme-linked immunoassay kit
Catalog# EM0038
Mouse LIF enzyme-linked immunoassay kit
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ELISA
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Mouse
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6.86~5000 pg/ml
Overview
Product Name
Mouse LIF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.77 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0337 | 0.0309 | 0.0323 | |
| 6.86 | 0.0458 | 0.0440 | 0.0449 | 0.0126 |
| 20.58 | 0.0698 | 0.0664 | 0.0681 | 0.0358 |
| 61.73 | 0.1404 | 0.1345 | 0.1375 | 0.1052 |
| 185.19 | 0.3507 | 0.3391 | 0.3449 | 0.3126 |
| 555.56 | 0.9113 | 0.9009 | 0.9061 | 0.8738 |
| 1666.67 | 2.1080 | 2.0780 | 2.0930 | 2.0607 |
| 5000.00 | 3.6630 | 3.7060 | 3.6845 | 3.6522 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 102.3 | 501.8 | 1489.4 | 100.7 | 491.3 | 1511.1 |
| Standard Deviation | 3.2 | 20.1 | 62.0 | 3.6 | 15.2 | 44.2 |
| Coefficient of Variation (%) | 3.1 | 4.0 | 4.2 | 3.5 | 3.1 | 2.9 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠LIF，未加小鼠的LIF血清作为本底，计算回收率。回收率的范围从 94 % 至 105 %，平均回收率为 100 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 133.17-389.78 | 100 | 261.5 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CDF
Cholinergic Differentiation Factor
D factor
DIA
Differentiation inducing factor
differentiation inhibitory activity
Differentiation stimulating factor
Differentiation-stimulating factor
Emfilermin
Hepatocyte stimulating factor III
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