Mouse Adiponectin enzyme-linked immunoassay kit(one step)
Catalog# EMY0064
Mouse Adiponectin enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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1.37~1000 ng/ml
Overview
Product Name
Mouse Adiponectin enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.25 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0070 | 0.0073 | 0.0072 | |
| 1.37 | 0.0102 | 0.0103 | 0.0103 | 0.0031 |
| 4.12 | 0.0159 | 0.0162 | 0.0161 | 0.0089 |
| 12.35 | 0.0354 | 0.0358 | 0.0356 | 0.0285 |
| 37.04 | 0.1021 | 0.0822 | 0.0922 | 0.0850 |
| 111.11 | 0.2987 | 0.2578 | 0.2783 | 0.2711 |
| 333.33 | 0.8970 | 0.7846 | 0.8408 | 0.8337 |
| 1000.00 | 2.4410 | 2.2860 | 2.3635 | 2.3564 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 13.5 | 75.9 | 218.3 | 22.6 | 105.6 | 316.4 |
| Standard Deviation | 0.9 | 5.8 | 13.3 | 0.4 | 2.6 | 7.1 |
| Coefficient of Variation (%) | 6.7 | 7.6 | 6.1 | 1.9 | 2.4 | 2.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 Adiponectin，未加小鼠 Adiponectin 的血清作为本底，计算回收率。回收率的范围从 80 % 至 119 %，平均回收率为 105 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.61-27.96 | 100 | 15.44 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
30 kDa adipocyte complement related protein
30 kDa adipocyte complement-related protein
ACDC
Acrp 30
ACRP30
ADIPO_HUMAN
Adipocyte
Adipocyte C1q and collagen domain containing protein
Adipocyte complement related 30 kDa protein
Adipocyte complement related protein of 30 kDa
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