Mouse Periostin/POSTN enzyme-linked immunoassay kit
Catalog# EM0075
Mouse Periostin/POSTN enzyme-linked immunoassay kit
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ELISA
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Mouse
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156.25 ~10000 pg/ml
Overview
Product Name
Mouse Periostin/POSTN enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
5.93 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0172 | 0.0157 | 0.0165 | |
| 156.25 | 0.0644 | 0.0643 | 0.0644 | 0.0479 |
| 312.50 | 0.1121 | 0.1146 | 0.1134 | 0.0969 |
| 625.00 | 0.2067 | 0.2147 | 0.2107 | 0.1943 |
| 1250.00 | 0.4168 | 0.4183 | 0.4176 | 0.4011 |
| 2500.00 | 0.8173 | 0.8154 | 0.8164 | 0.7999 |
| 5000.00 | 1.6130 | 1.6290 | 1.6210 | 1.6046 |
| 10000.00 | 2.9470 | 2.9890 | 2.9680 | 2.9516 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 199.0 | 1022.8 | 3131.7 | 204.4 | 995.0 | 2988.8 |
| Standard Deviation | 9.3 | 44.6 | 138.9 | 9.2 | 34.9 | 120.4 |
| Coefficient of Variation (%) | 4.7 | 4.4 | 4.4 | 4.5 | 3.5 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse Periostin/POSTN into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 113% with an overall mean recovery of 91%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.88-10.18 | 100 | 7.37 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of mouse Periostin/POSTN in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃