Mouse IFN alpha-1 enzyme-linked immunoassay kit
Catalog# EM0049
Mouse IFN alpha-1 enzyme-linked immunoassay kit
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ELISA
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Mouse
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78.13~5000 pg/ml
Overview
Product Name
Mouse IFN alpha-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.65 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0255 | 0.0288 | 0.0272 | |
| 78.13 | 0.1023 | 0.1111 | 0.1067 | 0.0796 |
| 156.25 | 0.1886 | 0.1889 | 0.1888 | 0.1616 |
| 312.50 | 0.3705 | 0.3604 | 0.3655 | 0.3383 |
| 625.00 | 0.7418 | 0.7227 | 0.7323 | 0.7051 |
| 1250.00 | 1.4051 | 1.4527 | 1.4289 | 1.4018 |
| 2500.00 | 2.7303 | 2.5847 | 2.6575 | 2.6304 |
| 5000.00 | 4.0742 | 4.1814 | 4.1278 | 4.1007 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 87.84 | 491.57 | 1544.40 | 88.73 | 480.46 | 1517.12 |
| Standard Deviation | 4.99 | 18.68 | 66.80 | 2.53 | 15.37 | 57.77 |
| Coefficient of Variation (%) | 5.7 | 3.8 | 4.3 | 2.8 | 3.2 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IFN alpha-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 98% with an overall mean recovery of 96%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-26.30 | 66.7 | 13.29 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IFN alpha-1 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
IFL
IFN alpha
IFN alpha 1/13
IFN alpha 1b
IFN alphaD
IFN
IFN-alpha-1/13
IFNA@
IFNA1
IFNA1_HUMAN
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