Mouse Insulin enzyme-linked immunoassay kit(one step)
Catalog# EMY0067
Mouse Insulin enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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78.13~5000 pg/ml
Overview
Product Name
Mouse Insulin enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
33.46 pg/ml (20 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0201 | 0.0179 | 0.0190 | |
| 78.13 | 0.0324 | 0.0323 | 0.0324 | 0.0134 |
| 156.25 | 0.0503 | 0.0460 | 0.0482 | 0.0292 |
| 312.50 | 0.0788 | 0.0922 | 0.0855 | 0.0665 |
| 625.00 | 0.2258 | 0.2212 | 0.2235 | 0.2045 |
| 1250.00 | 0.6657 | 0.7631 | 0.7144 | 0.6954 |
| 2500.00 | 1.9100 | 2.2820 | 2.0960 | 2.0770 |
| 5000.00 | 2.8400 | 3.0830 | 2.9615 | 2.9425 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 581.0 | 2316.1 | 7446.9 | 579.8 | 2277.0 | 7736.2 |
| Standard Deviation | 12.8 | 34.2 | 243.7 | 14.4 | 34.5 | 230.5 |
| Coefficient of Variation (%) | 2.2 | 1.5 | 3.3 | 2.5 | 1.5 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse Insulin into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 110%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1144.00 | 93.3 | 368.21 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Insulin in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃