Mouse Leptin enzyme-linked immunoassay kit
Catalog# EM0027
Mouse Leptin enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
13.72~10000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Mouse Leptin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.24 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0170 | 0.0179 | 0.0175 | |
| 13.72 | 0.0655 | 0.0633 | 0.0644 | 0.0470 |
| 41.15 | 0.1510 | 0.1464 | 0.1487 | 0.1313 |
| 123.46 | 0.3596 | 0.3765 | 0.3681 | 0.3506 |
| 370.37 | 0.9335 | 0.9369 | 0.9352 | 0.9178 |
| 1111.11 | 2.0700 | 2.0207 | 2.0454 | 2.0279 |
| 3333.33 | 3.2890 | 3.2855 | 3.2873 | 3.2698 |
| 10000.00 | 3.8812 | 4.0509 | 3.9661 | 3.9486 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 180.9 | 1004.4 | 3082.4 | 174.2 | 1023.2 | 3039.1 |
| Standard Deviation | 7.4 | 50.0 | 202.9 | 6.5 | 53.0 | 190.8 |
| Coefficient of Variation (%) | 4.1 | 5.0 | 6.6 | 3.8 | 5.2 | 6.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠Leptin，未加小鼠Leptin 的血清作为本底，计算回收率。回收率的范围从 97 % 至 103 %，平均回收率为 99 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1419.67-3710.99 | 100 | 4633.39 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
FLJ94114
LEP
LEP_HUMAN
LEPD
Leptin (murine obesity homolog)
Leptin (obesity homolog
mouse)
Leptin
Leptin Murine Obesity Homolog
Leptin Precursor Obesity Factor
ExpandFunction
瘦素是小鼠肥胖基因的产物。具有阻断瘦素合成的肥胖基因突变的小鼠肥胖和糖尿病，并且活动，代谢和体温降低。大鼠瘦素分别与小鼠和人类蛋白质共享约96%和82%的序列同一性。瘦素mRNA的表达仅限于脂肪组织。