Mouse IL-17A enzyme-linked immunoassay kit
Catalog# EM0008
Mouse IL-17A enzyme-linked immunoassay kit
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ELISA
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Mouse
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4.12~3000 pg/ml
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unconjugated
Overview
Product Name
Mouse IL-17A enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.39 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0084 | 0.0077 | 0.0081 | |
| 4.12 | 0.0150 | 0.0143 | 0.0147 | 0.0066 |
| 12.35 | 0.0299 | 0.0300 | 0.0300 | 0.0219 |
| 37.04 | 0.0777 | 0.0786 | 0.0782 | 0.0701 |
| 111.11 | 0.2320 | 0.2267 | 0.2294 | 0.2213 |
| 333.33 | 0.6311 | 0.6392 | 0.6352 | 0.6271 |
| 1000.00 | 1.6010 | 1.6680 | 1.6345 | 1.6265 |
| 3000.00 | 3.3530 | 3.5300 | 3.4415 | 3.4335 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 40.2 | 254.7 | 867.4 | 43.0 | 235.5 | 947.6 |
| Standard Deviation | 1.9 | 8.7 | 39.6 | 1.7 | 7.6 | 44.7 |
| Coefficient of Variation (%) | 4.8 | 3.4 | 4.6 | 3.9 | 3.2 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-17A into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 115% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.95-11.76 | 100 | 5.45 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-17A in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Mouse | See 1 publications below |
Target
UNIPROT
Alias
CTLA 8
CTLA-8
CTLA8
Cytotoxic T lymphocyte associated antigen 8
Cytotoxic T lymphocyte associated protein 8
Cytotoxic T lymphocyte associated serine esterase 8
Cytotoxic T-lymphocyte-associated antigen 8
IL 17A
IL-17
IL-17A
ExpandFunction
Citation
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Jianpi Fuzheng Xiaoji Formula Ameliorates Gastric Precancerous Lesions via Suppression of Neutrophil Extracellular Trap Formation
Journal: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2026.121459
IF: 5.4
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Mar