Mouse RANKL/TRANCE/TNFSF11 enzyme-linked immunoassay kit
Catalog# EM0044
Mouse RANKL/TRANCE/TNFSF11 enzyme-linked immunoassay kit
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ELISA
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Mouse
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2.74~2000 pg/ml
Overview
Product Name
Mouse RANKL/TRANCE/TNFSF11 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.36 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0138 | 0.0142 | 0.0140 | |
| 2.74 | 0.0234 | 0.0247 | 0.0241 | 0.0101 |
| 8.23 | 0.0475 | 0.0485 | 0.0480 | 0.0340 |
| 24.69 | 0.1120 | 0.1176 | 0.1148 | 0.1008 |
| 74.07 | 0.3230 | 0.3205 | 0.3218 | 0.3078 |
| 222.22 | 0.9004 | 0.8719 | 0.8862 | 0.8722 |
| 666.67 | 2.2855 | 2.2587 | 2.2721 | 2.2581 |
| 2000.00 | 4.1051 | 3.9973 | 4.0512 | 4.0372 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 60.20 | 211.59 | 633.72 | 62.06 | 191.99 | 568.45 |
| Standard Deviation | 3.50 | 8.89 | 30.71 | 3.23 | 10.65 | 28.61 |
| Coefficient of Variation (%) | 5.8 | 4.2 | 4.8 | 5.2 | 5.5 | 5.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 RANKL/TRANCE/TNFSF11，未加小鼠 RANKL/TRANCE/TNFSF11 的血清作为本底，计算回收率。回收率的范围从 90 % 至 111 %，平均回收率为 100 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 61.89-141.84 | 100 | 107.38 |
应用本试剂盒，检测 30 只健康小鼠的血清样本，小鼠的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD254
hRANKL2
ODF
OPGL
OPTB2
Osteoclast differentiation factor
Osteoprotegerin ligand
Rank Ligand
RANKL
Receptor activator of nuclear factor kappa B ligand
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