Mouse CCL3/MIP-1 alpha enzyme-linked immunoassay kit
Catalog# EM0031
Mouse CCL3/MIP-1 alpha enzyme-linked immunoassay kit
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ELISA
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Mouse
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1.37~1000 pg/ml
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unconjugated
Overview
Product Name
Mouse CCL3/MIP-1 alpha enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.51 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0159 | 0.0141 | 0.0150 | |
| 1.37 | 0.0232 | 0.0210 | 0.0221 | 0.0071 |
| 4.12 | 0.0341 | 0.0302 | 0.0322 | 0.0172 |
| 12.35 | 0.0651 | 0.0658 | 0.0655 | 0.0505 |
| 37.04 | 0.1703 | 0.1707 | 0.1705 | 0.1555 |
| 111.11 | 0.4507 | 0.4318 | 0.4413 | 0.4263 |
| 333.33 | 1.2730 | 1.2200 | 1.2465 | 1.2315 |
| 1000.00 | 3.1870 | 3.2800 | 3.2335 | 3.2185 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 18.8 | 88.6 | 247.5 | 18.5 | 96.2 | 282.2 |
| Standard Deviation | 1.4 | 3.9 | 13.2 | 1.2 | 6.7 | 10.1 |
| Coefficient of Variation (%) | 7.3 | 4.4 | 5.3 | 6.6 | 6.9 | 3.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的人CCL3，未加人 CCL3 的血清作为本底，计算回收率。回收率的范围从 106 % 至 139 %，平均回收率为 128 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.2-20.8 | 100 | 4.19 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C C motif chemokine 3
CCL 3
CCL3
CCL3_HUMAN
Chemokine C C motif ligand 3
G0/G1 switch regulatory protein 19 1
G0/G1 switch regulatory protein 19-1
G0S19 1
G0S19 1 protein
Heparin binding chemotaxis protein
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