Mouse IL1RL1/ST2 enzyme-linked immunoassay kit
Catalog# EM0077
Mouse IL1RL1/ST2 enzyme-linked immunoassay kit
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ELISA
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Mouse
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31.25 ~2000 pg/ml
Overview
Product Name
Mouse IL1RL1/ST2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.28 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0339 | 0.0310 | 0.0325 | |
| 31.25 | 0.0898 | 0.0932 | 0.0915 | 0.0591 |
| 62.50 | 0.1522 | 0.1544 | 0.1533 | 0.1209 |
| 125.00 | 0.2739 | 0.2948 | 0.2844 | 0.2519 |
| 250.00 | 0.5177 | 0.5427 | 0.5302 | 0.4978 |
| 500.00 | 0.9743 | 1.0340 | 1.0042 | 0.9717 |
| 1000.00 | 1.7840 | 1.9370 | 1.8605 | 1.8281 |
| 2000.00 | 3.0810 | 3.2760 | 3.1785 | 3.1461 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 50.4 | 228.8 | 663.4 | 57.2 | 245.0 | 675.8 |
| Standard Deviation | 2.1 | 9.3 | 28.1 | 1.5 | 6.8 | 28.9 |
| Coefficient of Variation (%) | 4.1 | 4.1 | 4.2 | 2.7 | 2.8 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL1RL1/ST2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 103% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 487.89-837.47 | 100 | 652.19 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of mouse IL1RL1/ST2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃