Mouse CD68/SR-D1 enzyme-linked immunoassay kit
Catalog# EM0042
Mouse CD68/SR-D1 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
78.13~5000 pg/ml
Overview
Product Name
Mouse CD68/SR-D1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.62 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0409 | 0.0415 | 0.0412 | |
| 78.13 | 0.1598 | 0.1527 | 0.1563 | 0.1151 |
| 156.25 | 0.2903 | 0.2588 | 0.2746 | 0.2334 |
| 312.50 | 0.5202 | 0.4834 | 0.5018 | 0.4606 |
| 625.00 | 0.9210 | 0.8673 | 0.8942 | 0.8530 |
| 1250.00 | 1.6190 | 1.6390 | 1.6290 | 1.5878 |
| 2500.00 | 2.9040 | 2.9550 | 2.9295 | 2.8883 |
| 5000.00 | 4.2213 | 4.1789 | 4.2001 | 4.1589 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 141.4 | 654.7 | 1898.7 | 129.9 | 593.6 | 1759.2 |
| Standard Deviation | 5.9 | 35.1 | 107.5 | 7.6 | 34.7 | 80.1 |
| Coefficient of Variation (%) | 4.2 | 5.4 | 5.7 | 5.8 | 5.8 | 4.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 CD68/SR-D1，未加小鼠 CD68/SR-D1 的血清作为本底，计算回收率。回收率的范围从 80 % 至 119 %，平均回收率为 95%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1438.48-2557.23 | 100 | 1971.16 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD 68
CD68
CD68 antigen
CD68 molecule
CD68_HUMAN
DKFZp686M18236
gp11
Gp110
LAMP4
Macrophage antigen CD68 (microsialin)
ExpandFunction
CD68，也称为清道夫受体 D1 （SR-D1），是一种 I 型跨膜糖蛋白，属于 LAMP 分子家族。CD68 在单核细胞和巨噬细胞上表达，可作为氧化 LDL 的清除剂受体。