Mouse CXCL16 enzyme-linked immunoassay kit
Catalog# EM0078
Mouse CXCL16 enzyme-linked immunoassay kit
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ELISA
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Mouse
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156.25~10000 pg/ml
Overview
Product Name
Mouse CXCL16 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
16.89 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0076 | 0.0073 | 0.0075 | |
| 156.25 | 0.0234 | 0.0247 | 0.0241 | 0.0166 |
| 312.50 | 0.0524 | 0.0516 | 0.0520 | 0.0446 |
| 625.00 | 0.1304 | 0.1323 | 0.1314 | 0.1239 |
| 1250.00 | 0.3880 | 0.3645 | 0.3763 | 0.3688 |
| 2500.00 | 1.0480 | 0.9918 | 1.0199 | 1.0125 |
| 5000.00 | 2.4590 | 2.4000 | 2.4295 | 2.4221 |
| 10000.00 | 4.0733 | 4.2712 | 4.1723 | 4.1648 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 194.0 | 958.5 | 2989.1 | 206.8 | 956.7 | 3140.6 |
| Standard Deviation | 7.8 | 31.8 | 91.6 | 8.3 | 20.1 | 109.3 |
| Coefficient of Variation (%) | 4.0 | 3.3 | 3.1 | 4.0 | 2.1 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL16 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 110% with an overall mean recovery of 90%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1656.93-3095.84 | 100 | 2014.13 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL16 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃