Mouse Osteopontin/OPN enzyme-linked immunoassay kit（one step）
Catalog# EMY0073
Mouse Osteopontin/OPN enzyme-linked immunoassay kit（one step）
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ELISA
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Mouse
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0.69~500 ng/ml
Overview
Product Name
Mouse Osteopontin/OPN enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.18 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0036 | 0.0035 | 0.0035 | |
| 0.69 | 0.0082 | 0.0078 | 0.0080 | 0.0045 |
| 2.06 | 0.0231 | 0.0227 | 0.0229 | 0.0194 |
| 6.17 | 0.0776 | 0.0765 | 0.0771 | 0.0736 |
| 18.52 | 0.2525 | 0.2577 | 0.2551 | 0.2516 |
| 55.56 | 0.7773 | 0.8046 | 0.7910 | 0.7875 |
| 166.67 | 2.0170 | 2.0230 | 2.0200 | 2.0165 |
| 500.00 | 3.5410 | 3.5850 | 3.5630 | 3.5595 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 13.8 | 59.2 | 186.5 | 10.6 | 51.2 | 175.9 |
| Standard Deviation | 0.3 | 2.3 | 7.3 | 0.4 | 1.7 | 7.6 |
| Coefficient of Variation (%) | 2.5 | 3.9 | 3.9 | 3.8 | 3.4 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse Osteopontin/OPN into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 112% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 46.96-265.49 | 100 | 117.02 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃