Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit(one step)
Catalog# EMY0045
Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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0.27~200 ng/ml
Overview
Product Name
Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.01 pg/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0059 | 0.0063 | 0.0061 | |
| 0.27 | 0.0196 | 0.0204 | 0.0200 | 0.0139 |
| 0.82 | 0.0474 | 0.0530 | 0.0502 | 0.0441 |
| 2.47 | 0.1412 | 0.1520 | 0.1466 | 0.1405 |
| 7.41 | 0.4025 | 0.4208 | 0.4117 | 0.4056 |
| 22.22 | 1.1140 | 1.2170 | 1.1655 | 1.1594 |
| 66.67 | 2.8660 | 2.8730 | 2.8695 | 2.8634 |
| 200.00 | 4.1954 | 4.3502 | 4.2728 | 4.2667 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 5.4 | 26.4 | 74.1 | 4.0 | 21.4 | 79.3 |
| Standard Deviation | 0.4 | 2.1 | 4.3 | 0.2 | 0.4 | 4.1 |
| Coefficient of Variation (%) | 6.7 | 7.8 | 5.8 | 4.8 | 2.0 | 5.1 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 Pro-collagen I alpha 1/COL1A1，未加小鼠 Pro-collagen I alpha 1/COL1A1 的血清作为本底，计算回收率。回收率的范围从 86 % 至 119 %，平均回收率为 103 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 146.01-320.89 | 100 | 223.31 |
应用本试剂盒，检测 30 只健康小鼠的血清样本，小鼠的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Alpha 1 type I collagen
alpha1(I) procollagen
CO1A1_HUMAN
COL1A1
collagen alpha 1 chain type I
Collagen alpha-1(I) chain
collagen alpha-1(I) chain preproprotein
Collagen I alpha 1 polypeptide
collagen of skin, tendon and bone, alpha-1 chain
Collagen type I alpha 1
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